Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/113423
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Benedé, Sara | - |
dc.contributor.author | Pérez-Rangel, I. | - |
dc.contributor.author | Lozano-Ojalvo, Daniel | - |
dc.contributor.author | Molina, Elena | - |
dc.contributor.author | Ibáñez, M. D. | - |
dc.contributor.author | López-Fandiño, Rosina | - |
dc.contributor.author | López-Expósito, Iván | - |
dc.date.accessioned | 2015-04-09T10:16:34Z | - |
dc.date.available | 2015-04-09T10:16:34Z | - |
dc.date.issued | 2014 | - |
dc.identifier | doi: 10.1159/000366101 | - |
dc.identifier | issn: 1018-2438 | - |
dc.identifier | e-issn: 1423-0097 | - |
dc.identifier.citation | International Archives of Allergy and Immunology 165(2): 83-90 (2014) | - |
dc.identifier.uri | http://hdl.handle.net/10261/113423 | - |
dc.description.abstract | [Background]: This paper reports the case of an egg-allergic pediatric patient who, once desensitized to egg following a successful rush oral immunotherapy protocol, could also tolerate Lizipaina®, a drug containing lysozyme (LYS) and papain, which had previously caused him a severe allergic reaction. Because the LYS amount that elicited the anaphylactic reaction (5 mg) was much lower than that tolerated during a double-blind placebo-controlled food challenge (corresponding to approximately 60 mg of LYS), the possibility that the presence of papain could increase the allergenic potential of LYS was investigated. [Methods]: Lizipaina, LYS and LYS hydrolyzed with papain were analyzed by SDS-PAGE under reducing and nonreducing conditions, and Western blotting of sera from egg-allergic patients was performed in order to detect IgE-binding fragments. Finally, sequence identification of the IgE-reactive bands was carried out by MALDI-TOF/TOF. [Results]: The SDS-PAGE pattern of LYS treated with papain under nonreducing conditions showed the presence of intact LYS that partially disappeared following reduction with β-mercaptoethanol, releasing IgE-reactive fragments as determined by Western blotting. MALDI-TOF/TOF revealed that papain degraded LYS, giving rise to three IgE-binding fragments: LYS (22-129), LYS (34-96) and LYS (62-128) that likely remained linked through the disulfide bonds present in the LYS molecule. Conclusion: The combined administration of LYS with proteolytic enzymes such as papain may have developed a severe allergic reaction in the patient studied, underlining the importance of considering all the components and their interactions when drugs are to be consumed by allergic persons. | - |
dc.description.sponsorship | The authors acknowledge financial support from Fundación Salud 2000 (Merck-Serono, Spain) and ALK-Abelló Laboratories (Madrid, Spain), and by the project AGL2011-24740. S. B. and I.L.-E. acknowledge the financial support of CSIC through JAE-Pre and JAE-Doc grants, and D.L.-O. the financial support of the Ministerio de Educación, Cultura y Deporte through a FPU grant. | - |
dc.publisher | S. Karger AG | - |
dc.rights | closedAccess | - |
dc.title | Anaphylaxis induced by a drug containing lysozyme and papain: Influence of papain on the IgE response | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1159/000366101 | - |
dc.date.updated | 2015-04-09T10:16:35Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Fundación Salud 2000 | - |
dc.contributor.funder | ALK-Abelló Laboratories | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Ministerio de Educación, Cultura y Deporte (España) | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007043 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003176 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
Aparece en las colecciones: | (CIAL) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
5
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
4
checked on 26-feb-2024
Page view(s)
338
checked on 17-abr-2024
Download(s)
104
checked on 17-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.