Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/113233
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Human midbrain precursors activate the expected developmental genetic program and differentiate long-term to functional A9 dopamine neurons in vitro. Enhancement by Bcl-X L |
Autor: | Seiz, Emma G.; Ramos Gómez, Milagros; Courtois, Elise T.; Tonnesen, Jan; Kokaia, Merab; Liste Noya, Isabel; Martínez-Serrano, Alberto CSIC ORCID | Palabras clave: | Neural development Parkinson’s disease cell therapy Bcl-XL Neuron maturation Floor plate Substantia nigra precursors |
Fecha de publicación: | 2012 | Editor: | Academic Press | Citación: | Experimental Cell Research 318: 2446- 2459 (2012) | Resumen: | Understanding the molecular programs of the generation of human dopaminergic neurons (DAn) from their ventral mesencephalic (VM) precursors is of key importance for basic studies, progress in cell therapy, drug screening and pharmacology in the context of Parkinson's disease. The nature of human DAn precursors in vitro is poorly understood, their properties unstable, and their availability highly limited. Here we present positive evidence that human VM precursors retaining their genuine properties and long-term capacity to generate A9 type Substantia nigra human DAn (hVM1 model cell line) can be propagated in culture. During a one month differentiation, these cells activate all key genes needed to progress from pro-neural and pro-dopaminergic precursors to mature and functional DAn. For the first time, we demonstrate that gene cascades are correctly activated during differentiation, resulting in the generation of mature DAn. These DAn have morphological and functional properties undistinguishable from those generated by VM primary neuronal cultures. In addition, we have found that the forced expression of Bcl-X L induces an increase in the expression of key developmental genes (MSX1, NGN2), maintenance of PITX3 expression temporal profile, and also enhances genes involved in DAn long-term function, maintenance and survival (EN1, LMX1B, NURR1 and PITX3). As a result, Bcl-X L anticipates and enhances DAn generation. © 2012 Elsevier Inc. | URI: | http://hdl.handle.net/10261/113233 | DOI: | 10.1016/j.yexcr.2012.07.018 | Identificadores: | doi: 10.1016/j.yexcr.2012.07.018 issn: 0014-4827 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
12
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
11
checked on 24-feb-2024
Page view(s)
323
checked on 24-abr-2024
Download(s)
100
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.