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dc.contributor.authorGago, Selma-
dc.contributor.authorElena, Santiago F.-
dc.contributor.authorFlores, Ricardo-
dc.contributor.authorSanjuán, Rafael-
dc.date.accessioned2009-03-06T11:43:24Z-
dc.date.available2009-03-06T11:43:24Z-
dc.date.issued2009-03-06-
dc.identifier.citationScience 323(5919): 1308 (2009)en_US
dc.identifier.issn0036-8075-
dc.identifier.urihttp://hdl.handle.net/10261/11315-
dc.descriptionSupporting information (Materials and methods, figs. S1-S3, suppl. references) available at: http://www.sciencemag.org/cgi/data/323/5919/1308/DC1/1-
dc.description.abstractThe mutation rates of viroids, plant pathogens with minimal non-protein-coding RNA genomes, are unknown. Their replication is mediated by host RNA polymerases and, in some cases, by hammerhead ribozymes, small self-cleaving motifs embedded in the viroid. By using the principle that the population frequency of nonviable genotypes equals the mutation rate, we screened for changes that inactivated the hammerheads of Chrysanthemum chlorotic mottle viroid. We obtained a mutation rate of 1/400 per site, the highest reported for any biological entity. Such error-prone replication can only be tolerated by extremely simple genomes such as those of viroids and, presumably, the primitive replicons of the RNA world. Our results suggest that the emergence of replication fidelity was critical for the evolution of complexity in the early history of life.en_US
dc.format.extent918459 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Association for the Advancement of Scienceen_US
dc.rightsclosedAccessen_US
dc.subjectViroidsen_US
dc.subjectChrysanthemum chlorotic mottle viroiden_US
dc.subjectMutation ratesen_US
dc.subjectHammerhead ribozymesen_US
dc.subjectRNA replicationen_US
dc.subjectReplication fidelityen_US
dc.titleExtremely high mutation rate of a hammerhead viroiden_US
dc.typeartículoen_US
dc.identifier.doihttp://dx.doi.org/10.1126/science.1169202-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1126/science.1169202en_US
dc.identifier.e-issn1095-9203-
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