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Título

A premature termination of human epidermal growth factor receptor transcription in Escherichia coli

Autor Elloumi-Mseddi, Jihene; Jellali, Karim; Villalobo, Antonio ; Aifa, Sami
Fecha de publicación 2014
EditorHindawi Publishing Corporation
Citación The Scientific World Journal: 830923 (2014)
ResumenOur success in producing an active epidermal growth factor receptor (EGFR) tyrosine kinase in Escherichia coli encouraged us to express the full-length receptor in the same host. Despite its large size, we were successful at producing the full-length EGFR protein fused to glutathione S-transferase (GST) that was detected by Western blot analysis. Moreover, we obtained a majoritarian truncated GST-EGFR form detectable by gel electrophoresis and Western blot. This truncated protein was purified and confirmed by MALDI-TOF/TOF analysis to belong to the N-terminal extracellular region of the EGFR fused to GST. Northern blot analysis showed two transcripts suggesting the occurrence of a transcriptional arrest.
Versión del editorhttp://dx.doi.org/10.1155/2014/830923
URI http://hdl.handle.net/10261/113142
DOI10.1155/2014/830923
Identificadoresdoi: 10.1155/2014/830923
issn: 2356-6140
e-issn: 1537-744X
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