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Título

Initiation signals for the conversion of single-stranded to double-stranded DNA forms in the streptococcal plasmid pLS1

Autor Solar, Gloria del ; Puyet, A.; Espinosa, Manuel
Fecha de publicación 1987
EditorOxford University Press
Citación Nucl. Acids Res. (1987) 15 (14): 5561-5580
ResumenWe have characterized a region in the streptococcal plasmid pLSl located between nucleotides 4103 and 4218 which is a signal involved in the conversion of single stranded intermediates of replication to double stranded plasmid forms. This region has a large axis of dyad symmetry resulting in the formation of a secondary structure as revealed by the location of endonuclease S1-cleavage sites in supercoiled covalently closed circular pLS1 DNA. Deletions affecting this region caused a fivefold reduction in plasmid copy number, plasmid instability and the accumulation of single-stranded DNA intermediates. The conversion signal of pLS1 has homologues in other staphylococcal plasmids, sharing a consensus sequence located in the loop of the signal. Computer assisted analysis showed that the signal detected in pLS1 has a high degree of homology with the complementary strand origin of the Escherichia coli single stranded bacteriophages øX174 and M13.
Descripción 20 p.-8 fig.-1 tab.
Versión del editorhttp://dx.doi.org/10.1093/nar/15.14.5561
URI http://hdl.handle.net/10261/112877
DOI10.1093/nar/15.14.5561
ISSN0305-1048
E-ISSN1362-4962
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