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Título

Serotonin 5-HT4 receptors and their mRNAs in rat and Guinea pig brain: Distribution and effects of neurotoxic lesions

Autor Vilaró, Maria Teresa ; Cortés, Roser ; Mengod Los Arcos, Guadalupe
Palabras clave Quinolinic acid
Striato-nigral pathway
In situ hybridization histochemistry
Presynaptic receptors
C-terminal splice variants
Striato-pallidal pathway
Fecha de publicación 15-mar-2005
EditorJohn Wiley & Sons
Citación Journal of Comparative Neurology 484(4): 418-439 (2005)
ResumenSerotonin 5-HT4 receptors are widely distributed in the periphery and in brain, where they modulate the release of various neurotransmitters and have been implicated in learning and memory. Nine C-terminal splice variants of this receptor have been cloned in mammalian species. In the rat, three such variants have been described: 5-HT 4(a), 5-HT4(b), and 5-HT4(e). In the present study, we have examined several aspects of the distribution of these receptors in brain. First, we provide, in rat and guinea pig, a detailed comparison of the distribution of 5-HT4 receptors labeled by the antagonist [ 125I]-SB 207710 with the distribution of their encoding mRNA visualized by in situ hybridization histochemistry (ISHH). The results suggest that, in several projection systems (striato-nigral and striato-pallidal pathways, projection from dentate granule cells to field CA3, habenulo-interpeduncular pathway), 5-HT4 receptors are located both somatodendritically and axonally. Second, we have analyzed the distribution of mRNA for the three known rat splice variants by reverse transcription-polymerase chain reaction (RT-PCR) and by ISHH. RT-PCR indicates that all three variants are widely distributed, with 5-HT4(b) mRNA being present in all regions examined (olfactory tubercle, striatum, hippocampus, inferior colliculus, substantia nigra, parietal cortex) and 5-HT4(a) and 5-HT4(e) showing a somewhat more restricted distribution. In other regions (periaqueductal gray, reticular formation, medial septum, diagonal band), faint ISHH signals are observed for 5-HT4(a)+4(e) mRNAs, whereas 5-HT4(b) mRNA signals are almost undetectable. Finally, neurotoxic lesions of basal ganglia components in guinea pig also indicate a location of these receptors on terminals of striatal projection neurons. © 2005 Wiley-Liss, Inc.
Versión del editorhttp://dx.doi.org/10.1002/cne.20447
URI http://hdl.handle.net/10261/112735
DOI10.1002/cne.20447
Identificadoresdoi: 10.1002/cne.20447
issn: 0021-9967
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