English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/11273
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 34 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Escherichia coli genes affecting recipient ability in plasmid conjugation: Are there any?

AutorPérez-Mendoza, Daniel; Cruz, Fernando de la
Fecha de publicación9-feb-2009
EditorBioMed Central
CitaciónBMC Genomics 10: 71 (2009)
Resumen[Background] How does the recipient cell contribute to bacterial conjugation? To answer this question we systematically analyzed the individual contribution of each Escherichia coli gene in matings using plasmid R388 as a conjugative plasmid. We used an automated conjugation assay and two sets of E. coli mutant collections: the Keio collection (3,908 E. coli single-gene deletion mutants) and a collection of 20,000 random mini-Tn10::Km insertion mutants in E. coli strain DH5α. The combined use of both collections assured that we screened > 99% of the E. coli non-essential genes in our survey.
[Results] Results indicate that no non-essential recipient E. coli genes exist that play an essential role in conjugation. Mutations in the lipopolysaccharide (LPS) synthesis pathway had a modest effect on R388 plasmid transfer (6 – 32% of wild type). The same mutations showed a drastic inhibition effect on F-plasmid transfer, but only in liquid matings, suggesting that previously isolated conjugation-defective mutants do in fact impair mating pair formation in liquid mating, but not conjugative DNA processing or transport per se.
[Conclusion] We conclude from our genome-wide screen that recipient bacterial cells cannot avoid being used as recipients in bacterial conjugation. This is relevant as an indication of the problems in curbing the dissemination of antibiotic resistance and suggests that conjugation acts as a pure drilling machine, with little regard to the constitution of the recipient cell.
Descripción14 pages, 6 figures, 3 tables.
Versión del editorhttp://dx.doi.org/10.1186/1471-2164-10-71
URIhttp://hdl.handle.net/10261/11273
DOI10.1186/1471-2164-10-71
ISSN1471-2164
Aparece en las colecciones: (IBBTEC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Escherichia coli gene.pdf480,9 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.