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Título

Neuronal expression of cAMP-specific phosphodiesterase 7B mRNA in the rat brain

AutorReyes-Irisarri, Elisabet ; Pérez-Torres, Silvia; Mengod Los Arcos, Guadalupe
Palabras claveCholinergic cells
PDE
Glutamatergic cells
In situ hybridization
GABAergic cells
Fecha de publicación2005
EditorElsevier
CitaciónNeuroscience 132(4): 1173-1185 (2005)
ResumencAMP plays an important role as second messenger molecule controlling multiple cellular processes in the brain. cAMP levels depend critically on the phosphodiesterases (PDE) activity, enzymes responsible for the clearance of intracellular cAMP. We have examined the regional distribution and cellular localization of mRNA coding for the cAMP-specific phosphodiesterase 7B (PDE7B) in rat brain by in situ hybridization histochemistry. PDE7B mRNA is specifically distributed in rat brain, preferentially in neuronal cell populations. The highest levels of hybridization are observed in olfactory tubercle, islands of Calleja, dentate gyrus, caudate-putamen and some thalamic nuclei. Positive hybridization signals are also detected in other areas, such as cerebral cortex, Purkinje cells of the cerebellum and area postrema. By double in situ hybridization histochemistry, we found that 74% and 79% of the cells expressing PDE7B mRNA in striatum and olfactory tubercle, respectively, were GABAergic cells (expressing glutamic acid decarboxylase mRNA), in contrast with the lack of expression in the few cholinergic cells (expressing choline acetyltransferase mRNA) present in those two areas (around 0.4% in olfactory tubercle). In the thalamic nuclei, a majority of cells containing PDE7B mRNA also expresses a glutamatergic marker (76.7% express vesicular glutamate transporter vGluT1 and 76% express vGluT2 mRNAs). Almost all PDE7B expressing cells in dentate gyrus (93%) were glutamatergic. These results offer a neuroanatomical and neurochemical base that will support the search for specific functions for cAMP dependent PDEs and for the development of specific PDE7 inhibitors. © 2005 IBRO. Published by Elsevier Ltd. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.neuroscience.2005.01.050
URIhttp://hdl.handle.net/10261/112693
DOI10.1016/j.neuroscience.2005.01.050
Identificadoresdoi: 10.1016/j.neuroscience.2005.01.050
issn: 0306-4522
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