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Título

Conjugated linoleic acid in ewe milk fat

Autor Luna, Pilar ; Fontecha, F. Javier ; Juárez, Manuela ; Fuente, Miguel Ángel de la
Palabras clave Ag+-HPLC.
DMOX
GC-MS
fatty acid
CLA
milk
Ewe
Fecha de publicación 2005
EditorCambridge University Press
Citación Journal of Dairy Research 72: 415- 424 (2005)
ResumenEwe milk fat from five different herds was studied to determine the content of conjugated linoleic acid (CLA) isomers. Research was carried out by combining gas chromatography-mass spectrometry (GC-MS) of fatty acid methyl esters (FAME) and 4,4-dimethyloxazolyne derivatives (DMOX) with silver ion-high performance liquid chromatography (Ag+-HPLC). Reconstructed mass spectral profiles of CLA characteristic ions from DMOX were used to identify positional isomers and Ag+-HPLC to quantify them. Total CLA content varied from 0.57 to 0.97 g/100 g of total fatty acids. FAME and DMOX were separated into a complex mixture of minor isomers and major rumenic acid (9-cis 11-trans C18:2) by GC-MS using a 100-m polar capillary column. Rumenic acid would represent more than 75% of total CLA. 11-trans 13-trans, 11-13 cis/trans plus trans/cis and 7-9 cis/trans plus trans/cis were the main CLA isomers after rumenic acid. Minor amounts of 8-10 and 10-12 C18:2 isomers were also found. Although most of the isomers were present in each herd's milk, differences in content were observed for some CLA species. © Proprietors of Journal of Dairy Research 2005.
URI http://hdl.handle.net/10261/112614
DOI10.1017/S0022029905001032
Identificadoresdoi: 10.1017/S0022029905001032
issn: 0022-0299
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