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Title

Functional mapping of the GAGA factor assigns its transcriptional activity to the C-terminal glutamine-rich domain

AuthorsVaquero, Alejandro; Espinàs, Maria Lluïsa ; Azorín, Ferran ; Bernués, Jordi
Issue Date30-Jun-2000
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 275(26): 19461-19468 (2000)
AbstractGAGA is a nuclear protein encoded by the Trithorax-like gene in Drosophila that is expressed in at least two isoforms generated by alternative splicing. By means of its specific interaction with DNA, GAGA has been involved in several nuclear transactions including regulation of gene expression. Here we have studied the GAGA519 isoform as a transcription factor. In vitro, the transactivation domain has been assigned to the 93 C- terminal residues that correspond to a glutamine-rich domain (Q-domain). It presents an internal modular structure and acts independently of the rest of the protein. In vivo, in Drosophila SL2 cells, Q-domain can transactivate reporter genes either in the form of GAGA or Gal4BD-Q fusions, whereas a GAGA mutant deleted of the Q-domain cannot. Our results give support to the notion that GAGA can function as a transcription activating factor.
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M000967200
URIhttp://hdl.handle.net/10261/112466
DOI10.1074/jbc.M000967200
Identifiersdoi: 10.1074/jbc.M000967200
issn: 0021-9258
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