English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/112466
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 28 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Functional mapping of the GAGA factor assigns its transcriptional activity to the C-terminal glutamine-rich domain

Autor Vaquero, Alejandro; Espinàs, Maria Lluïsa ; Azorín, Ferran ; Bernués, Jordi
Fecha de publicación 30-jun-2000
EditorAmerican Society for Biochemistry and Molecular Biology
Citación Journal of Biological Chemistry 275(26): 19461-19468 (2000)
ResumenGAGA is a nuclear protein encoded by the Trithorax-like gene in Drosophila that is expressed in at least two isoforms generated by alternative splicing. By means of its specific interaction with DNA, GAGA has been involved in several nuclear transactions including regulation of gene expression. Here we have studied the GAGA519 isoform as a transcription factor. In vitro, the transactivation domain has been assigned to the 93 C- terminal residues that correspond to a glutamine-rich domain (Q-domain). It presents an internal modular structure and acts independently of the rest of the protein. In vivo, in Drosophila SL2 cells, Q-domain can transactivate reporter genes either in the form of GAGA or Gal4BD-Q fusions, whereas a GAGA mutant deleted of the Q-domain cannot. Our results give support to the notion that GAGA can function as a transcription activating factor.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M000967200
URI http://hdl.handle.net/10261/112466
DOI10.1074/jbc.M000967200
Identificadoresdoi: 10.1074/jbc.M000967200
issn: 0021-9258
Aparece en las colecciones: (CID) Artículos
(IBMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
jbc.M000967200.full-2.pdf1,88 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.