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Correction: Direct Sequencing from the Minimal Number of DNA Molecules Needed to Fill a 454 Picotiterplate

AutorDžunková, Mária; Garcia-Garcerà, Marc ; Martínez-Priego, Llucia ; D’Auria, Giussepe; Calafell, Francesc ; Moya-Simarro, Andrés
Fecha de publicación8-jul-2014
EditorPublic Library of Science
CitaciónPLoS ONE 9(6): e97379 (2014)
ResumenThe large amount of DNA needed to prepare a library in next generation sequencing protocols hinders direct sequencing of small DNA samples. This limitation is usually overcome by the enrichment of such samples with whole genome amplification (WGA), mostly by multiple displacement amplification (MDA) based on Q29 polymerase. However, this technique can be biased by the GC content of the sample and is prone to the development of chimeras as well as contamination during enrichment, which contributes to undesired noise during sequence data analysis, and also hampers the proper functional and/or taxonomic assignments. An alternative to MDA is direct DNA sequencing (DS), which represents the theoretical gold standard in genome sequencing. In this work, we explore the possibility of sequencing the the notion of one-bead-one-molecule. Using an optimized protocol for DS, we constructed a shotgun library containing the minimum number of DNA molecules needed to fill a selected region of a picotiterplate. We gathered most of the reference genome extension with uniform coverage. We compared the DS method with MDA applied to the same amount of starting DNA. As expected, MDA yielded a sparse and biased read distribution, with a very high amount of unassigned and unspecific DNA amplifications. The optimized DS protocol allows unbiased sequencing to be performed from samples with a very small amount of DNA.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0102719
URIhttp://hdl.handle.net/10261/112211
DOI10.1371/journal.pone.0097379
ISSN1932-6203
ReferenciasDžunková, Mária; García-Garcerà, Marc; Martínez-Priego, Llúcia; D’Auria, Giussepe; Calafell, Francesc; Moya, Andrés. Direct squencing from the minimal number of DNA molecules needed to fill a 454 picotiterplate. http://dx.doi.org/10.1371/journal.pone.0097379 . http://hdl.handle.net/10261/112205
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