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Título

Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120

AutorOlmedo-Verd, E. ; Valladares, Ana ; Flores, Enrique ; Herrero, Antonia ; Muro-Pastor, Alicia M.
Fecha de publicación2008
EditorAmerican Society for Microbiology
CitaciónJournal of Bacteriology 190: 7584- 7590 (2008)
ResumenAnabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N2 in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
URIhttp://hdl.handle.net/10261/111847
DOI10.1128/JB.00856-08
Identificadoresdoi: 10.1128/JB.00856-08
issn: 0021-9193
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