English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/11182
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Characterization of adult Dicrocoelium dendriticum by isoelectric focusing.

AuthorsCampo, Raquel; Manga-González, M. Yolanda ; González Lanza, Camino ; Rollinson, D.; Sandoval, Hilda
Issue Date1998
PublisherCambridge University Press
CitationJournal of Helminthology 72(2): 109-116 (1998)
AbstractWater soluble extracts of 3131 adult specimens of Dicrocoelium dendriticum from cattle, sheep and goats, mainly from León province, were analysed by isoelectric focusing in thin-layer polyacrylamide gels. Activity of the following enzymes was studied: lactate dehydrogenase (LDH, EC, glucose phosphate isomerase (GPI, EC, phosphoglucomutase (PGM, EC, acid phosphatase (AcP, EC, alpha-glycerophosphate dehydrogenase (alpha-GPDH, EC, hydroxybutyrate dehydrogenase (HBDH, EC and malate dehydrogenase (MDH, Five distinct enzyme types were recognized for LDH (pH range 6.30-7.13), GPI (pH 6.13-6.80) and PGM (pH 6.20-6.60) whereas AcP showed three different patterns (pH 5.70-5.92). Weak and diffuse activity was detected for MDH (pH 4.8-6.2) and no activity was observed for alpha-GPDH and HBDH. In general, little phenotypic variation was observed between worms recovered from a single host, between those from hosts of the same species and between those from hosts of different species, although some enzyme types were found in some animals but not others. Nevertheless, it must be taken into account that most parasites came from sheep and also from a relatively small area in north-west Spain.
Appears in Collections:(IGM) Artículos
Files in This Item:
File Description SizeFormat 
Journal of Helminthology 1998.pdf920,24 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.