English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/111759
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC7120

AuthorsElhai, Jeff; Vepritskiy, A.; Muro-Pastor, Alicia M. ; Flores, Enrique ; Wolk, C. Peter
Issue Date1997
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 179: 1998- 2005 (1997)
AbstractThe efficiency of conjugal transfer of plasmids from Escherichia coli to the cyanobacterium Anabaena sp. strain PCC 7120 was quantitated as a function of the number of restriction sites for the restriction enzymes carried by the recipient. In addition to the previously recognized isoschizomers of AvaI and AvaII, PCC 7120 was found to possess an isoschizomer of AvaIII. Plasmids modified in E. coli with methylases that protect in vitro against restriction by the three enzymes were transferred with high efficiency, nearly independent of the number of restriction sites on the plasmid. Plasmids left unprotected against one of the three restriction enzymes were transferred with lower efficiencies. For low numbers of sites, the efficiency of conjugal transfer decreased as an exponential function of the number of unprotected sites. The methods presented may be used to increase the efficiency of conjugal transfer into restriction-competent bacteria.
URIhttp://hdl.handle.net/10261/111759
Identifiersissn: 0021-9193
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
J. Bacteriol.-1997-Elhai-1998-2005.pdf837,44 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.