English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/11171
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 29 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Rga2 IS a Rho2 GAP that regulates morphogenesis and cell integrity in Schizosaccharomyces pombe

AutorVillar-Tajadura, María Antonia; Coll, Pedro M.; Santos Romero, Beatriz; Pérez González, Pilar
Palabras claveGTPase
Rho
GAP
Morphogenesis
Cell integrity
Fecha de publicación10-oct-2008
EditorBlackwell Publishing
CitaciónMolecular Microbiology 70(4): 867–881 (2008)
ResumenSchizosaccharomyces pombe Rho2 GTPase regulates alpha-D-glucan synthesis and acts upstream of Pck2 to activate the MAP kinase pathway for cell integrity. However, little is known about its regulation. Here we describe Rga2 as a Rho2 GTPase-activating protein (GAP) that regulates cell morphology. rga2+ gene is not essential for growth but its deletion causes longer and thinner cells whereas rga2+ overexpression causes shorter and broader cells. rga2+ overexpression also causes abnormal accumulation of Calcofluor-stained material and cell lysis, suggesting that it also participates in cell wall integrity. Rga2 localizes to growth tips and septum region. The N-terminal region of the protein is required for its correct localization whereas the PH domain is necessary exclusively for Rga2 localization to the division area. Also, Rga2 localization depends on polarity markers and on actin polymerization. Rga2 interacts with Rho2 and possesses in vitro and in vivo GAP activity for this GTPase. Accordingly, rga2Delta cells contain more alpha-D-glucan and therefore partially suppress the thermosensitivity of mok1-664 cells, which have a defective alpha-D-glucan synthase. Additionally, genetic interactions and biochemical analysis suggest that Rga2 regulates Rho2-Pck2 interaction and might participate in the regulation of the MAPK cell integrity pathway.
Descripción15 pages, 8 figures.-- PMID: 18793338 [PubMed].-- Supporting information (Table S1: List of strains used in the study) available at: http://www3.interscience.wiley.com/journal/121408738/suppinfo
Versión del editorhttp://dx.doi.org/10.1111/j.1365-2958.2008.06447.x
URIhttp://hdl.handle.net/10261/11171
DOI10.1111/j.1365-2958.2008.06447.x
ISSN0950-382X (Print)
1365-2958 (Online)
Aparece en las colecciones: (IMB) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.