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Title

Combination of different microscopy techniques for the integrated study of extremophile endolithic microorganisms and their habitats

AuthorsRíos, Asunción de los ; Wierzchos, Jacek ; Sancho, Leopoldo G.; Ascaso, Carmen
Keywordsextreme environments
microecosystems
SEM_BSE
LTSEM
CSLM
EDS
lithobiontic
biomobilisation
Issue DateAug-2001
PublisherEuropean Space Agency
CitationExo-/Astro-Biology : proceedings of the first european workshop 21 - 23 May 2001, ESRIN, Frascati, Italy / editors P. Ehrenfreund, O. Angerer and B. Battrick.
SeriesESA SP
496: 405-408(2001)
AbstractSome micro-organisms are able to withstand extreme environments and form communities within rocks. To characterise these endolithic microecosystems, several microscopy and microanalytical approaches need to be combined, including scanning electron microscopy with back scattered imaging(SEM-BSE), low temperature scanning electron microscopy (LTSEM), confocal scanning laser microscopy (CSLM), and the X-ray energy dispersive spectroscopy (EDS) microanalytical system. These techniques have alowed the simultaneous observation of these micro-organisms and their habitats. SEM-BSE and LTSEM serves to evaluate the biodiversity of the rock from a morphological and ultrastructural perspective LTSEM also permits water localisation in the cells and their microhabitats. Information on the spatial distribution of the micro-organisms inside the rock is provided by CSLM.
Description4 páginas y 7 figuras.
Publisher version (URL)http://adsabs.harvard.edu/abs/2001ESASP.496..405D
URIhttp://hdl.handle.net/10261/111632
ISBN92-9092-806-9
E-ISSN0379-6566
Appears in Collections:(CCMA) Comunicaciones congresos
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