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Divergent role of ceramide generated by exogenous sphingomyelinases on NF-κB activation and apoptosis in human colon HT-29 cells

AuthorsColell Riera, Anna ; Coll, Olga; Marí, Montserrat ; Fernández-Checa, José C. ; García-Ruiz, Carmen
Transcription nuclear factor-κB
Issue Date2002
CitationFEBS Letters 526(1-3): 15-20 (2002)
AbstractThis study examined the role of ceramide generated by exogenous sphingomyelinases (SMases) on transcription nuclear factor-κB (NF-κB) activation and apoptosis in human colon epithelial HT-29 cells. Exogenous neutral (N) and acidic (A) SMase activated NF-κB with different kinetics, accounting for the diverse pattern of DNA binding of NF-κB complexes activated by tumor necrosis factor-α (TNF). NSMase activated predominantly RelA/p52 and RelA/p50 dimers within 30 min, while ASMase activated the p50/p50 homodimer by 20 h. The predominant activation of RelA-containing κB complexes by TNF or NSMase paralleled the induction of interleukin-8. HT-29 cells were sensitive to ASMase and TNF but resistant to NSMase. However, the apoptotic potential of NSMase was masked by NF-κB, as its prior inactivation sensitized HT-29 cells to NSMase. Thus, the generation of ceramide by exogenous SMases participates differentially in inflammation and apoptosis. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Publisher version (URL)http://dx.doi.org/10.1016/S0014-5793(02)03106-X
Identifiersdoi: 10.1016/S0014-5793(02)03106-X
issn: 0014-5793
Appears in Collections:(IIBB) Artículos
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