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Determination of tetracycline antibiotic residues in edible swine tissues by liquid chromatography with spectrofluorometric detection and confirmation by mass spectrometry

AuthorsPena, Angelina; Lino, Maria Celeste; Alonso Díez, Rosa María; Barceló, Damià
Swine tissues
Liquid chromatography
Spectrofluorometric detection
Mass spectrometry detection
Issue Date6-Jun-2007
PublisherAmerican Chemical Society
CitationJournal of Agriculture and Food Chemistry 55(13): 4973-4979 (2007)
AbstractA sensitive and specific method is described for the simultaneous determination of oxytetracycline, tetracycline (TC), and chlortetracycline residues in edible swine tissues, by combining liquid chromatography with spectrofluorometric and mass spectrometry detection. The procedure involved a preliminary extraction with EDTA-McIlvaine buffer acidified at pH 4.0, followed by solid-phase extraction cleanup using a polymeric sorbent. The liquid chromatography analysis was performed with spectrofluorometric detection after postcolumn derivatization with magnesium ions. The limits of quantification were 50 μg/kg for muscle and 100 μg/kg for kidney tissues. The recovery values were greater than 77.8% for muscle and 65.1% for kidney. The method has been successfully used for the quantification of tetracyclines in swine tissues samples. The selective liquid chromatography mass spectrometric analysis for confirmation of oxytetracycline in one positive swine muscle sample was made by atmospheric pressure chemical ionization (APCI). The APCI mass spectra of the TCs gave the protonated molecular ion and two typical fragment ions, required for their confirmation in single ion monitoring scan mode in animal tissues.
Description7 pages, 2 tables, 5 figures.-- PMID: 17550268 [PubMed].-- Printed version published on Jun 27, 2007.
Publisher version (URL)http://dx.doi.org/10.1021/jf070398j
Appears in Collections:(IDAEA) Artículos
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