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Título: | Analysis of neofructooligosaccharides production mediated by the extracellular β-fructofuranosidase from Xanthophyllomyces dendrorhous |
Autor: | Linde, Dolores CSIC ORCID ; Rodríguez-Colinas, Bárbara CSIC; Estévez, Marta; Poveda, Ana; Plou Gasca, Francisco José CSIC ORCID ; Fernández Lobato, María CSIC ORCID | Palabras clave: | Neofructooligosaccharides Xanthophyllomyces dendrorhous Extracellular b-fructofuranosidase Prebiotics |
Fecha de publicación: | 2012 | Editor: | Elsevier | Citación: | Bioresource Technology 109: 123- 130 (2012) | Resumen: | The extracellular β-fructofuranosidase Xd-INV from the yeast Xanthophyllomyces dendrorhous mainly synthesizes the neo-fructooligosaccharides (neo-FOS) neokestose and neonystose. This enzyme is a glycoprotein with a content of 59-67% N-linked carbohydrates and an estimated molecular mass of 160-200. kDa. The extent level of glycosylation affects the thermal behaviour of the enzyme but not its hydrolase and transferase activities, which are optimal at 60-70 °C. The neo-FOS yield of this enzyme was increased from 40 to 168. g/L when the sucrose concentration increased from 420 to 600. g/L and when the reaction was carried out at 60 °C. The neo-FOS levels obtained (168. g/L) in this work are the largest reported for any microbial β-fructofuranosidase. © 2012 Elsevier Ltd. | URI: | http://hdl.handle.net/10261/111450 | DOI: | 10.1016/j.biortech.2012.01.023 | Identificadores: | doi: 10.1016/j.biortech.2012.01.023 issn: 0960-8524 |
Aparece en las colecciones: | (CBM) Artículos (ICP) Artículos |
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