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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Morín, José Antonio | - |
dc.contributor.author | Cao, Francisco J. | - |
dc.contributor.author | Lázaro, José M. | - |
dc.contributor.author | Arias-Gonzalez, J. Ricardo | - |
dc.contributor.author | Valpuesta, José M. | - |
dc.contributor.author | Carrascosa, José L. | - |
dc.contributor.author | Salas, Margarita | - |
dc.contributor.author | Ibarra, Borja | - |
dc.date.accessioned | 2015-02-24T10:16:19Z | - |
dc.date.available | 2015-02-24T10:16:19Z | - |
dc.date.issued | 2012 | - |
dc.identifier | doi: 10.1073/pnas.1204759109 | - |
dc.identifier | issn: 0027-8424 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109: 8115- 8120 (2012) | - |
dc.identifier.uri | http://hdl.handle.net/10261/111105 | - |
dc.description.abstract | Duplication of double-stranded DNA (dsDNA) requires a fine-tuned coordination between the DNA replication and unwinding reactions. Using optical tweezers, we probed the coupling dynamics between these two activities when they are simultaneously carried out by individual Phi29 DNA polymerase molecules replicating a dsDNA hairpin. We used the wild-type and an unwinding deficient polymerase variant and found that mechanical tension applied on the DNA and the DNA sequence modulate in different ways the replication, unwinding rates, and pause kinetics of each polymerase. However, incorporation of pause kinetics in a model to quantify the unwinding reaction reveals that both polymerases destabilize the fork with the same active mechanism and offers insights into the topological strategies that could be used by the Phi29 DNA polymerase and other DNA replication systems to couple unwinding and replication reactions. | - |
dc.description.sponsorship | BFU2011-29038- CO2-1 and CSD2007-00010; BFU2010-15703/BMC; BFU2008-00215/BMC; CSD2007-00015; RYC2007-01765; S2009MAT-1507 ; FIS2010-17440 GR35/10-A-920GR35/10-A-911 | - |
dc.publisher | National Academy of Sciences (U.S.) | - |
dc.rights | openAccess | - |
dc.subject | Molecular motors | - |
dc.subject | Single-molecule | - |
dc.subject | Strad displacement | - |
dc.subject | Replicative DNA plymerase | - |
dc.title | Active DNA unwinding dynamics during processive DNA replication | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1073/pnas.1204759109 | - |
dc.date.updated | 2015-02-24T10:16:20Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | Instituto de Salud Carlos III | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.pmid | 22573817 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
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M_Salas_Active_DNA.pdf | 1,1 MB | Adobe PDF | Visualizar/Abrir |
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