English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/111053
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 10 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Crystallization and preliminary X-ray analysis of NADP(H)-dependent alcohol dehydrogenases from Saccharomyces cerevisiae and Rana perezi

Autor Valencia, Eva; Rosell, A.; Larroy, Carolina; Farrés, Jaume; Biosca, Josep Antoni; Fita, Ignacio ; Parés, Xavier; Ochoa, Wendy F.
Fecha de publicación 1-feb-2003
EditorBlackwell Publishing
Citación Acta Crystallographica Section D: Biological Crystallography 59(2): 334-337 (2003)
ResumenDifferent crystal forms diffracting to high resolution have been obtained for two NADP(H)-dependent alcohol dehydrogenases, members of the medium-chain dehydrogenase/reductase superfamily: ScADHVI from Saccharomyces cerevisiae and ADH8 from Rana perezi. ScADHVI is a broad-specificity enzyme, with a sequence identity lower than 25% with respect to all other ADHs of known structure. The best crystals of ScADHVI diffracted beyond 2.8 resolution and belonged to the trigonal space group P3121 (or to its enantiomorph P3221), with unit-cell parameters a = b = 102.2, c = 149.7 Å, γ = 120°. These crystals were produced by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. Packing considerations together with the self-rotation function and the native Patterson map seem to indicate the presence of only one subunit per asymmetric unit, with a volume solvent content of about 80%. ADH8 from R. perezi is the only NADP(H)-dependent ADH from vertebrates characterized to date. Crystals of ADH8 obtained both in the absence and in the presence of NADP+ using polyethylene glycol and lithium sulfate as precipitants diffracted to 2.2 and 1.8 Å, respectively, using synchrotron radiation. These crystals were isomorphous, space group C2, with approximate unit-cell parameters a = 122, b = 79, c = 91 Å, β = 113° and contain one dimer per asymmetric unit, with a volume solvent content of about 50%.
Versión del editorhttp://dx.doi.org/10.1107/S090744490201661X
URI http://hdl.handle.net/10261/111053
DOI10.1107/S090744490201661X
Identificadoresdoi: 10.1107/S090744490201661X
issn: 0907-4449
Aparece en las colecciones: (IBMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Valencia-Acta-D-2003-v59-n2-p334.pdf372,78 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.