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Título: | Determination of copper in different chloroplast preparations |
Autor: | Arellano, Juan B. CSIC ORCID ; Barón Ayala, Matilde CSIC ORCID; Chueca, Ana CSIC ; Lachica, M. CSIC | Palabras clave: | Chloroplast Copper determination Electrothermal atomic absorption spectrometry |
Fecha de publicación: | jul-1993 | Editor: | Springer Nature | Citación: | Plant and Soil (1993) 154:7-11 | Resumen: | The determination of total Cu is not often correlated with states of deficiency in plant material. This fact makes it necessary to look for biologically active Cu. Suspensions of thylakoid membranes and photosystem II particles, properly diluted with 13 mM nitric acid, were used for this purpose. The presence of a minute quantity of an antifoaming agent, such as 1-octanol, is essential when an aliquot of the slurry is injected into the graphite furnace of the atomic absorption spectrophotometer. Good agreement was obtained between our results and those obtained by a classical dry combustion method. Reproducibility was better than 5% when expressed as relative standard deviation. | Versión del editor: | http://www.springerlink.com/content/rv68r95110kw2720/ | URI: | http://hdl.handle.net/10261/11104 | DOI: | 10.1007/BF00011064 |
Aparece en las colecciones: | (IRNASA) Artículos (EEZ) Artículos |
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Arellano et al 1993.pdf | 142,41 kB | Adobe PDF | Visualizar/Abrir |
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