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Title

Determination of copper in different chloroplast preparations

AuthorsArellano, Juan B. CSIC ORCID ; Barón Ayala, Matilde; Chueca, Ana; Lachica Garrido, M.
KeywordsChloroplast
Copper determination
Electrothermal atomic absorption spectrometry
Issue DateJul-1993
PublisherSpringer
CitationPlant and Soil (1993) 154:7-11
AbstractThe determination of total Cu is not often correlated with states of deficiency in plant material. This fact makes it necessary to look for biologically active Cu. Suspensions of thylakoid membranes and photosystem II particles, properly diluted with 13 mM nitric acid, were used for this purpose. The presence of a minute quantity of an antifoaming agent, such as 1-octanol, is essential when an aliquot of the slurry is injected into the graphite furnace of the atomic absorption spectrophotometer. Good agreement was obtained between our results and those obtained by a classical dry combustion method. Reproducibility was better than 5% when expressed as relative standard deviation.
Publisher version (URL)http://www.springerlink.com/content/rv68r95110kw2720/
URIhttp://hdl.handle.net/10261/11104
DOI10.1007/BF00011064
Appears in Collections:(IRNASA) Artículos
(EEZ) Artículos

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