English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/11101
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
 |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations

AutorArellano, Juan B. ; Schröder, Wolfgang P.; Sandmann, Gerhard; Chueca, Ana; Barón Ayala, Matilde
Palabras clavePhotosystem II
Nuclear contaminants
Copper
Histones
Fecha de publicaciónjul-1994
EditorJohn Wiley & Sons
CitaciónPhysiol. Plant. (1994) 91:369-374.
ResumenIn conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N-terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non-specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch-free BBY (BBYs-), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII.
Versión del editorhttp://www3.interscience.wiley.com/journal/119267376/abstract
URIhttp://hdl.handle.net/10261/11101
DOI10.1111/j.1399-3054.1994.tb02962.x About DOI
Aparece en las colecciones: (IRNASA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Arellano et al 1994.pdf620,36 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.