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Gas Chromatographic Method for Analysis of Conjugated Linoleic Acids Isomers (c9t11, t10c12, and t9t11) in Broth Media as Application in Probiotic Studies

AuthorsAlonso López, Leocadio ; Cuesta, Paloma; Gilliland, S. E.
Issue Date2004
PublisherOxford University Press
CitationJournal of Chromatographic Science 42(3): 167-170 (2004)
AbstractA gas chromatography (GC) procedure is assayed for analysis of conjugated linoleic acid (CLA) isomers cis-9, trans-11-octadecadienoic (c9t11); trans-10, 12 cis-octadecadienoic (t10c12); and trans-9, trans-11-octadecadienoic (t9t11) in culture broth by GC using NaOH-BF3 in methanol for methylating and a long capillary (100 m) high-polarity column. Repeatability of the method is assessed; the coefficient of variation for CIA isomers ranges from 4.62 for c9t11 to 8.19 for t9t11. Recovery ranges between 88.01 and 89.76, with a mean value of 89.06 for all CLA isomers studied. This method may be considered advantageous for analysis of CLA isomers in probiotics cultures samples.
Publisher version (URL)http://dx.doi.org/10.1093/chromsci/42.3.167
Identifiersdoi: 10.1093/chromsci/42.3.167
issn: 0021-9665
e-issn: 1945-239X
Appears in Collections:(IPLA) Artículos
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