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dc.contributor.authorMejía-Ramírez, Eva-
dc.contributor.authorSánchez-Gorostiaga, Alicia-
dc.contributor.authorKrimer, Dora B.-
dc.contributor.authorSchvartzman, Jorge Bernardo-
dc.contributor.authorHernandez, Pablo-
dc.date.accessioned2015-02-19T09:12:07Z-
dc.date.available2015-02-19T09:12:07Z-
dc.date.issued2005-10-
dc.identifier.citationMol Cell Biol. 2005, 25(19): 8755–8761es_ES
dc.identifier.issn0270-7306-
dc.identifier.urihttp://hdl.handle.net/10261/110794-
dc.description25 p.-5 fig.-1 tab.es_ES
dc.description.abstractSchizosaccharomyces pombe rDNA contains three replication fork barriers (RFB1-3) located in the non-transcribed spacer. RFB2 and RFB3 require binding of the transcription terminator factor Reb1p to two identical recognition sequences that co-localize with these barriers. RFB1, which is the strongest of the three barriers, functions in a Reb1pindependent manner and cognate DNA-binding proteins for this barrier have not been identified yet. Here, we functionally defined RFB1 within a 78-bp sequence located near the 3’-end of the rDNA coding region. A protein that specifically binds to this sequence was purified by affinity chromatography and identified as Sap1p by mass spectrometry. Specific binding to RFB1 was confirmed by using Sap1p expressed in E. coli. Sap1p is essential for viability and is required for efficient mating-type switching. Mutations in RFB1 that precluded formation of the Sap1p-RFB1 complex systematically abolished replication barrier function, indicating that Sap1p is required for replication fork blockage at RFB1.es_ES
dc.description.sponsorshipThis work was partially supported by grants BFU2004-00125, SAF2001-1740 and BMC2002-00546 and a fellowship to E.M.-R, from the Spanish Ministerio de Educación y Ciencia, and a fellowship from the Comunidad de Madrid-Fondo Social Europeo to A.S.-G.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.titleThe mating-type-switch activating protein Sap1 is required for replication fork arrest at the rDNA of fission yeastes_ES
dc.title.alternativeSap1p and replication fork blockage in S. pombe rDNA-
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1128/MCB.25.19.8755-8761.2005-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1128/MCB.25.19.8755-8761.2005es_ES
dc.identifier.e-issn1098-5549-
dc.relation.csices_ES
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