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http://hdl.handle.net/10261/110694
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Vallejo, Carmen G. | - |
dc.contributor.author | Cruz-Bermúdez, Alberto | - |
dc.contributor.author | Clemente, Paula | - |
dc.contributor.author | Hernández-Sierra, Rosana | - |
dc.contributor.author | Garesse, Rafael | - |
dc.contributor.author | Quintanilla, Miguel | - |
dc.date.accessioned | 2015-02-16T11:05:51Z | - |
dc.date.available | 2015-02-16T11:05:51Z | - |
dc.date.issued | 2013 | - |
dc.identifier | doi: 10.1016/j.biochi.2013.01.001 | - |
dc.identifier | issn: 0300-9084 | - |
dc.identifier | e-issn: 1638-6183 | - |
dc.identifier.citation | Biochimie 95(6): 1171-1176 (2013) | - |
dc.identifier.uri | http://hdl.handle.net/10261/110694 | - |
dc.description.abstract | Metabolic reprogramming from mitochondrial aerobic respiration to aerobic glycolysis is a hallmark of cancer. However, whether it is caused by a dysfunction in the oxidative phosphorylation pathway is still under debate. In this work, we have analyzed the bioenergetic cellular (BEC) index and the relative cell ability to grow in the presence of either galactose or glucose as sources of sugar (Gal/Glu index) of a system formed by four epidermal cell lines with increasing tumorigenic potentials, ranging from nontumorigenic to highly malignant. We find that the BEC index gradually decreases whereas the Gal/Glu index increases with tumorigenicity, indicating that a progressive metabolic adaptation to aerobic glycolysis occurs in tumor cells associated with malignancy. Interestingly, this metabolic adaptation does not appear to be caused by damaged respiration, since the expression and activity of components of the respiratory chain complexes were unchanged in the cell lines. Moreover, the corresponding mitochondrial ATP synthetic abilities of the cell lines were found similar. The production of reactive oxygen species was also measured. A shift in ROS generation was found when compared nontumorigenic with tumorigenic cell lines, the latter exhibiting about threefold higher ROS levels than nontumorigenic cells. This result indicates that oxidative stress is an early event during tumor progression. © 2013 Elsevier Masson SAS. All rights reserved. | - |
dc.description.sponsorship | This work was supported by grants of the Center for Biomedical Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III (grants PI 07/0167 and PI 10/0703 to RG), Comunidad Autónoma de Madrid (grant number GEN-0269 to RG and grant S2010/BMD-2359, SkinModel-CM, to MQ) and the Spanish Ministry of Science and Innovation (grant SAF2010-19152 to MQ). | - |
dc.publisher | Elsevier | - |
dc.relation | S2010/BMD-2359/SKINMODEL | - |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | - |
dc.subject | ROS | - |
dc.subject | Carcinogenesis | - |
dc.subject | Metabolic reprogramming | - |
dc.subject | Glycolysis | - |
dc.subject | Mitochondria | - |
dc.title | Evaluation of mitochondrial function and metabolic reprogramming during tumor progression in a cell model of skin carcinogenesis | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.biochi.2013.01.001 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.biochi.2013.01.001 | - |
dc.date.updated | 2015-02-16T11:05:51Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Instituto de Salud Carlos III | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Centro de Investigación Biomédica en Red Enfermedades Raras (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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skin carcinogenesis.pdf | 1,13 MB | Adobe PDF | Visualizar/Abrir |
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