English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/110522
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Enhanced enzymatic activity of glycerol-3-phosphate dehydrogenase from the cryophilic Saccharomyces kudriavzevii

AuthorsOliveira, Bruno M.; Barrio, Eladio ; Querol, Amparo ; Pérez Torrado, Roberto
KeywordsS. kudriavzevii
Wine
Low temperature
Osmotic shock
Alcoholic Fermentation
Saccharomyces
Glycerol
Issue Date2014
PublisherPublic Library of Science
CitationPLoS ONE 9 (2014)
AbstractDuring the evolution of the different species classified within the Saccharomyces genus, each one has adapted to live in different environments. One of the most important parameters that have influenced the evolution of Saccharomyces species is the temperature. Here we have focused on the study of the ability of certain species as Saccharomyces kudriavzevii to grow at low temperatures, in contrast to Saccharomyces cerevisiae. We observed that S. kudriavzevii strains isolated from several regions are able to synthesize higher amounts of glycerol, a molecule that has been shown to accumulate in response to freeze and cold stress. To explain this observation at the molecular level we studied the expression of glycerol biosynthetic pathway genes and we observed a higher expression of GPD1 gene in S. kudriavzevii compared to S. cerevisiae in micro-vinification conditions. We observed higher enzymatic activity of Gpd1p in S. kudriavzevii in response to osmotic and cold stress. Also, we determined that S. kudriavzevii Gpd1p enzyme presents increased catalytic properties that will contribute to increase glycerol production. Finally, we evaluated the glycerol production with S. cerevisiae, S. kudriavzevii or a recombinant Gpd1p variant in the same background and observed that the S. kudriavzevii enzyme produced increased glycerol levels at 12 or 28°C. This suggests that glycerol is increased in S. kudriavzevii mainly due to increased Vmax of the Gpd1p enzyme. All these differences indicate that S. kudriavzevii has changed the metabolism to promote the branch of the glycolytic pathway involved in glycerol production to adapt to low temperature environments and maintain the NAD+/NADH ratio in alcoholic fermentations. This knowledge is industrially relevant due to the potential use, for example, of S. cerevisiae -S. kudriavzevii hybrids in the wine industry where glycerol content is an important quality parameter. © 2014 Oliveira et al.
URIhttp://hdl.handle.net/10261/110522
DOIhttp://dx.doi.org/10.1371/journal.pone.0087290
Identifiersdoi: 10.1371/journal.pone.0087290
issn: 1932-6203
Appears in Collections:(IATA) Artículos
Files in This Item:
File Description SizeFormat 
journal.pone.0087290.pdf819,04 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.