English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/110458
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Use of a metallomics approach to assess the biological response of the clam Scrobiculariaplana in several environmental areas of South West Iberian Peninsula

AuthorsGago Tinoco, Amanda; González-Fernández, M.; García-Barrera, Tamara; Blasco, Julián ; Bebianno, Maria João; Gómez-Ariza, José Luis
KeywordsMetallomics
Scrobicularia plana
Issue DateJan-2012
CitationXVI Seminario Ibérico de Química Marina (2012)
AbstractThe assessment of environmental issues is generally performed by the use of bioindicators that provide the biological response to contaminants. Mammals and invertebrates have been frequently proposed for this aim, and the study of conventional biomarkers supplied a deeper knowledge about the toxicological mechanisms. The great number of substances and metabolites involved in these complex mechanisms has driven the use of omics, which are the new tool to evaluate the global biological response to contamination. Metallomics is the most recent omic focused in the study of metal-binding biomolecules using instrumental couplings, generally based on the use of SEC-ICP-MS and organic mass spectrometry. In the present study we apply this approach to compare the biological response of Scrobicularia plana collected from polluted and non-polluted areas from the south-west of Spain and Portugal. Digestive gland of the clam was extracted to obtain the cytosolic fraction and the chromatographic profiles of the extracts reveal the presence of low molecular mass Cu and Zn molecules about 300 Da that can be related to the Cu- and Zn-glutathiones and As metabolites such as arsenobetaine and arsenocholine. The up-regulation of Cu-biomolecules is higher in Cadiz area against Portugal zone while Zn-biomolecules present an opposite behaviour. In several samples has been observed the presence of a remarkable Cu-peak with molecular mass of about 67 kDa. Metal biomolecules up- or down-regulated under the action of contaminants are being identified.
DescriptionTrabajo presentado en el XVI Seminario Ibérico de Química Marina, celebrado en Cádiz del 24 al 26 de enero de 2012.
URIhttp://hdl.handle.net/10261/110458
Appears in Collections:(ICMAN) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.