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Use of a metallomics approach to assess the biological response of the clam Scrobiculariaplana in several environmental areas of South West Iberian Peninsula

AutorGago Tinoco, Amanda; González-Fernández, M.; García-Barrera, Tamara; Blasco, Julián ; Bebianno, Maria João; Gómez-Ariza, José Luis
Palabras claveMetallomics
Scrobicularia plana
Fecha de publicaciónene-2012
CitaciónXVI Seminario Ibérico de Química Marina (2012)
ResumenThe assessment of environmental issues is generally performed by the use of bioindicators that provide the biological response to contaminants. Mammals and invertebrates have been frequently proposed for this aim, and the study of conventional biomarkers supplied a deeper knowledge about the toxicological mechanisms. The great number of substances and metabolites involved in these complex mechanisms has driven the use of omics, which are the new tool to evaluate the global biological response to contamination. Metallomics is the most recent omic focused in the study of metal-binding biomolecules using instrumental couplings, generally based on the use of SEC-ICP-MS and organic mass spectrometry. In the present study we apply this approach to compare the biological response of Scrobicularia plana collected from polluted and non-polluted areas from the south-west of Spain and Portugal. Digestive gland of the clam was extracted to obtain the cytosolic fraction and the chromatographic profiles of the extracts reveal the presence of low molecular mass Cu and Zn molecules about 300 Da that can be related to the Cu- and Zn-glutathiones and As metabolites such as arsenobetaine and arsenocholine. The up-regulation of Cu-biomolecules is higher in Cadiz area against Portugal zone while Zn-biomolecules present an opposite behaviour. In several samples has been observed the presence of a remarkable Cu-peak with molecular mass of about 67 kDa. Metal biomolecules up- or down-regulated under the action of contaminants are being identified.
DescripciónTrabajo presentado en el XVI Seminario Ibérico de Química Marina, celebrado en Cádiz del 24 al 26 de enero de 2012.
URIhttp://hdl.handle.net/10261/110458
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