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dc.contributor.authorQuirós, Laia-
dc.contributor.authorRaldúa, Demetrio-
dc.contributor.authorNavarro Cuenca, Anna-
dc.contributor.authorCasado, Marta-
dc.contributor.authorBarceló, Damià-
dc.contributor.authorPiña, Benjamín-
dc.date.accessioned2009-02-26T12:23:20Z-
dc.date.available2009-02-26T12:23:20Z-
dc.date.issued2007-10-
dc.identifier.citationEnvironmental Toxicology and Chemistry 26(10): 2179-2186 (2007)en_US
dc.identifier.issn0730-7268-
dc.identifier.urihttp://hdl.handle.net/10261/11040-
dc.description8 pages, 3 tables, 3 figures.-- PMID: 17867895 [PubMed].en_US
dc.description.abstractElevated expression of cytochrome P4501A (CYP1A) is an established biomarker for exposition to a wide range of toxicants, particularly for dioxin and structurally similar compounds. Expression of CYP1A usually is analyzed in internal organs, which involves dissection of the specimen. To avoid unnecessary animal killing, we present here an alternative method based on the monitoring of CYP1A expression in fish scales. Using β-naphthoflavone (BNF; 50 mg/kg body wt, intraperitoneal injection) as inducer in goldfish (Carassius auratus), we monitored levels of CYP1A mRNA both in scales and liver of treated and control specimens. Treatment with BNF resulted in a similar induction of CYP1A gene in both tissues, although scales responded faster (at 8 h after treatment) than liver (between 24 and 48 h). The scale-based test has the unique advantage of allowing sequential testing in the same specimen, which facilitates analysis of the time course of CYP1A induction and allows the study of individual variability. The method implies minimal suffering of the animals, because it only requires removal of a moderate (n = 1–3) number of scales for each time point. This nondestructive, fast, and relatively inexpensive test for toxic exposure therefore is suitable for environmental monitoring and food safety control programs in which specimen preservation is required.en_US
dc.description.sponsorshipThis work has been supported by the European Union and reflects only the author's views, and the European Community is not liable for any use that may be made of the information contained in it (AQUATERRA, GOCE-CT-2004-505428). The Spanish Ministry of Education and Science project GOCE-CT-2004-505428 (CTM2005-25168-E, CGL-2004-03514/HD, and BIO2005-00840) is also acknowledged for financial support. D. Raldúa acknowledges the "Ramón y Cajal" contract from the Spanish Ministry of Education and Science.en_US
dc.format.extent162 bytes-
dc.format.mimetypeapplication/msword-
dc.language.isoengen_US
dc.publisherSETAC (Society)en_US
dc.rightsclosedAccessen_US
dc.subjectQuantitative real-time polymerase chain reactionen_US
dc.subjectFish ecotoxicologyen_US
dc.subjectGenetic biomarkersen_US
dc.subjectEnvironmental biomonitoringen_US
dc.subjectAryl hydrocarbon receptoren_US
dc.titleA noninvasive test of exposition to toxicants: quantitative analysis of cytochrome P4501A expression in fish scalesen_US
dc.typeartículoen_US
dc.identifier.doi10.1897/07-027R.1-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1897/07-027R.1en_US
dc.identifier.e-issn1552-8618-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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