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Título

Simultaneous detection of two breast cancer-related miRNAs in tumor tissues using p19-based disposable amperometric magnetobiosensing platforms

Autor Torrente-Rodríguez, R.M.; Campuzano, Susana ; López-Hernández, E.; Montiel, V.R.; Barderas, Rodrigo ; Granados, R.; Sánchez-Puelles, José María ; Pingarrón, J.M.
Palabras clave miR-21
miR-205
p19
Simultaneous detection
Magnetic beads
SPdCEs
Cancer samples
Fecha de publicación 15-abr-2015
EditorElsevier
Citación Biosensors and Bioelectronics 66 (385-391) 2015
ResumenA novel magnetobiosensing approach for the rapid and simultaneous detection of two breast cancer-related miRs (miR-21 and miR-205) is reported. It involves the use of antimiR-21 and antimiR-205 specific probes, chitin-modified magnetic beads (Chitin-MBs), the p19 viral protein as capture bioreceptor and amperometric detection with the H2O2/hydroquinone (HQ) system at dual screen-printed carbon electrodes (SPCEs). The use of dual SPCEs allows the simultaneous independent amperometric readout for each target miR to be measured. The magnetosensor exhibited sensitive and selective detection of both target miRs with a dynamic range from 2.0 to 10.0 nM and detection limits of 0.6 nM (6 fmol) for both target miRs without any amplification step in less than 2 h. The usefulness of the approach was evaluated by detecting the endogenous levels of both target miRs in total RNA (RNAt) extracted from metastatic breast cancer cell lines and human tissues.
Descripción 23 p.-5 fig.1 tab.
Versión del editorhttp://dx.doi.org/ 10.1016/j.bios.2014.11.047
URI http://hdl.handle.net/10261/110380
DOI10.1016/j.bios.2014.11.047
ISSN0956-5663
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