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Título

Phytochelatins as biomarkers of cadmium and copper toxicity in marine microalgae

Autor Hampel, Miriam ; Moreno-Garrido, Ignacio ; Blasco, Julián
Palabras clave Heavy metals
Marine microalgae
Phytochelatins
Fecha de publicación may-2011
Citación PRIMO 16 (2011)
ResumenPhytochelatins (PCs) are detoxifying intracellular metal-binging peptides which are synthesized by microalgae as a response to metal stress. Marine microalgae are particularly promising indicator species for pollutants since they are typically the most abundant life forms in aquatic environments and occupy the base of the food chain. 72-h exposure toxicity tests with cadmium and copper were performed on four marine microalgae, namely Isochrysis galbana, Nannochloropsis gaditana, Phaeodactylum tricornutum and Rhodomonas salina. For all the four strains, copper EC50 values are much lower than for cadmium, confirming that copper is more toxic than cadmium to marine microalgae. Results of this study showed N. gaditana as the most tolerant to copper but also the most sensitive to cadmium whereas P. tricornutum and R. salina are the most tolerant to cadmium. In a second trial, the synthesis of PCs as a sublethal response to metal bioavailability in microalgae has been studied in laboratory cultures of the four selected marine microalgae exposed to the previously obtained cadmium and copper EC50 72-h values. Our bioassays showed to provoke a significant increase of PC synthesis in microalgae exposed to the metals, with the marine diatom P. tricornutum as the most sensitive species for this endpoint, and cadmium has shown to be more effective to induce PCs than copper in this specie.
Descripción Trabajo presentado en el PRIMO 16 (Pollutant Responses in Marine Organisms), celebrado en Long Beach (California, USA) del 15 al 18 de mayo de 2011.
URI http://hdl.handle.net/10261/110367
Aparece en las colecciones: (ICMAN) Comunicaciones congresos
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