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Título

Pepsin activity and pepsinogen mRNA expression during protein digestion in gilthead seabream juveniles

Autor Yúfera, Manuel ; Pujante Rodríguez, Isabel; Moyano, F. J.; Romero, María José; Mancera, Juan Miguel; Sánchez-Vázquez, F. J.; Martínez-Rodríguez, Gonzalo
Fecha de publicación jun-2012
Citación XV International Symposium on Fish Nutrition and Feeding (2012)
ResumenPepsin is activated from its precursor, the pepsinogen, at low gastric pH environment. In fish with stomach, low pH values are eventually attained during the postprandial period. Previous results indicated that meal timing and frequency affected the daily acidification rhythm. These gastric pH variations may affect the potential digestive capacity in relation to the pepsin activity. In this study we have examined the daily pepsin activity pattern in gilthead seabream Sparus aurata juveniles (5-7 g wet weight) maintained under 12L/12D photoperiod and three different feeding regimes: A) one daily meal at 9:00, B) two meals at 9:00 and 17:00, respectively, and C) continuous feeding during the light period, from 9:00 to 21:00. In all cases the same amount of food was supplied. Specifically we have determined the pepsin activity at the actual working pH and compared with the gene expression of mRNA codifying pepsinogen in samples taken periodically during a 24 h cycle.
Descripción Trabajo presentado en el XV International Symposium on Fish Nutrition and Feeding, celebrado en Molde (Noruega) del 4 al 7 de junio de 2012.
URI http://hdl.handle.net/10261/110365
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