Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/110279
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Respiratory complexes III and IV can each bind two molecules of cytochrome c at low ionic strength |
Autor: | Moreno-Beltrán, Blas CSIC ORCID; Díaz-Moreno, Irene CSIC ORCID; González-Arzola, Katiuska CSIC ORCID; Guerra-Castellano, Alejandra CSIC ORCID; Velázquez-Campoy, Adrián; Rosa, Miguel A. de la CSIC ORCID; Díaz-Quintana, Antonio | Palabras clave: | Cytochrome c Cytochrome bc1 Cytochrome c oxidase Isothermal Titration Calorimetry Nuclear magnetic resonance Supercomplex |
Fecha de publicación: | 2015 | Editor: | Elsevier | Citación: | FEBS Letters, 598(4): 476-483 (2015) | Resumen: | The transient interactions of respiratory cytochrome c with complexes III and IV is herein investigated by using heterologous proteins, namely human cytochrome c, the soluble domain of plant cytochrome c1 and bovine cytochrome c oxidase. The binding molecular mechanisms of the resulting cross-complexes have been analyzed by Nuclear Magnetic Resonance and Isothermal Titration Calorimetry. Our data reveal that the two cytochrome c-involving adducts possess a 2:1 stoichiometry – that is, two cytochrome c molecules per adduct – at low ionic strength. We conclude that such extra binding sites at the surfaces of complexes III and IV can facilitate the turnover and sliding of cytochrome c molecules and, therefore, the electron transfer within respiratory supercomplexes. | Versión del editor: | http://dx.doi.org/10.1016/j.febslet.2015.01.004 | URI: | http://hdl.handle.net/10261/110279 | DOI: | 10.1016/j.febslet.2015.01.004 |
Aparece en las colecciones: | (IBVF) Artículos (IQF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Moreno-Beltran_etal_FEBSLett.pdf | 9,77 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
14
checked on 12-mar-2024
SCOPUSTM
Citations
25
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
24
checked on 26-feb-2024
Page view(s)
311
checked on 23-abr-2024
Download(s)
469
checked on 23-abr-2024