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Molecular endocrine changes of GH/IGF-I axis in gilthead seabream (Sparus aurata) larvae exposed to different environmental salinity

Autor Geba, Khaled M.; Khallaf, A. G.; Yúfera, Manuel ; Martínez-Rodríguez, Gonzalo ; Mancera, Juan Miguel
Fecha de publicación sep-2011
Citación 8º Congreso de la Asociación Ibérica de Endocrinología Comparada (2011)
ResumenGilthead seabream (Sparus aurata) is a euryhaline teleost that favours estuaries for growing of larval and juvenile stages. Post-weaned larvae (n = 4000, 50 days old) were acclimated to two different environmental salinities (seawater, SW, 40¿; and brackish water, BW, 20¿) for 28 days, after which they were transferred to 40¿ salinity and acclimated for an additional week. Larvae (n=6/tank; each salinity by duplicate) were collected into RNAlater for Q-RT-PCR analysis. Expression changes in 8 genes encoding for hormones and neuropeptides intimately related to growth and osmoregulatory processes were assessed: Pituitary Adenylate Cyclase Activating Peptide (PACAP), Somatostatin-I( SSTI, Growth hormone (GH), Prolactin (PRL), Somatolactin SL, Pro-opiomelanocortines (POMC¿1 and POMC¿2), and Insulin-like Grwth Factor -I (IGF-I). PACAP, GH and IGF-I expression enhanced in BW-acclimated specimens, but not significant differences were observed for GH. However Sst-I expression was not affected by any salinity acclimation. PRL and SL presented an inverse response to environmental salinity, enhancing PRL expression in BW and SL under SW conditions. POMC¿1, POMC¿2 did not show significant changes in any tested groups. Our results indicated an enhancement of GH/IGF axis in BW-acclimated larvae, confirmed the role of PRL as a hypoosmotic promoting hormone, and suggested a role of SL in osmoregulatory processes as well as an equally low stress state between specimens acclimated to both environmental salinities. Acknowledgements: this work was supported by Aquagenomics CDS2007-00002 (MICINN, Spain, and FEDER/ERDF). KMG is supported by a doctoral fellowship from the Egyptian Government.
Descripción Trabajo presentado en el 8º Congreso de la Asociación Ibérica de Endocrinología Comparada (AIEC), celebrado en Madrid del 5 al 7 de septiembre de 2011.
URI http://hdl.handle.net/10261/110259
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