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Título

Mechanical stability and reversible fracture of vault particles

Autor Llauró, Aida; Guerra, Pablo; Irigoyen, Nerea; Rodríguez, José Francisco; Verdaguer, Núria ; Pablo, Pedro J. de
Fecha de publicación 4-feb-2014
EditorBiophysical Society
Citación Biophysical Journal 106(3): 687-695 (2014)
ResumenVaults are the largest ribonucleoprotein particles found in eukaryotic cells, with an unclear cellular function and promising applications as vehicles for drug delivery. In this article, we examine the local stiffness of individual vaults and probe their structural stability with atomic force microscopy under physiological conditions. Our data show that the barrel, the central part of the vault, governs both the stiffness and mechanical strength of these particles. In addition, we induce single-protein fractures in the barrel shell and monitor their temporal evolution. Our high-resolution atomic force microscopy topographies show that these fractures occur along the contacts between two major vault proteins and disappear over time. This unprecedented systematic self-healing mechanism, which enables these particles to reversibly adapt to certain geometric constraints, might help vaults safely pass through the nuclear pore complex and potentiate their role as self-reparable nanocontainers. © 2014 Biophysical Society.
Versión del editorhttp://dx.doi.org/10.1016/j.bpj.2013.12.035
URI http://hdl.handle.net/10261/110169
DOI10.1016/j.bpj.2013.12.035
Identificadoresdoi: 10.1016/j.bpj.2013.12.035
issn: 0006-3495
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