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Title

Structure of foot-and-mouth disease virus RNA-dependent RNA polymerase and its complex with a template-primer RNA

AuthorsFerrer-Orta, Cristina ; Arias, Armando ; Pérez-Luque, Rosa ; Escarmís, Cristina; Domingo, Esteban ; Verdaguer, Núria
Issue Date5-Nov-2004
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 279(45): 47212-47221 (2004)
AbstractGenome replication in picornaviruses is catalyzed by a virally encoded RNA-dependent RNA polymerase, termed 3D. The enzyme performs this operation, together with other viral and probably host proteins, in the cytoplasm of their host cells. The crystal structure of the 3D polymerase of foot-and-mouth disease virus, one of the most important animal pathogens, has been determined unliganded and bound to a template-primer RNA decanucleotide. The enzyme folds in the characteristic fingers, palm and thumb subdomains, with the presence of an NH2-terminal segment that encircles the active site. In the complex, several conserved amino acid side chains bind to the template-primer, likely mediating the initiation of RNA synthesis. The structure provides essential information for studies on RNA replication and the design of antiviral compounds.
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M405465200
URIhttp://hdl.handle.net/10261/110047
DOIhttp://dx.doi.org/10.1074/jbc.M405465200
Identifiersdoi: 10.1074/jbc.M405465200
issn: 0021-9258
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