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Title

RhaU of Rhizobium leguminosarum is a rhamnose mutarotase

AuthorsRichardson, Jason S.; Carpena, Xavi ; Switala, Jack; Pérez-Luque, Rosa ; Donald, Lynda J.; Loewen, Peter C.; Oresnik, Ivan J.
Issue DateApr-2008
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 190(8): 2903-2910 (2008)
AbstractOf the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a ΔrhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose into the cell and the rate of its phosphorylation were unaffected by the mutation. RhaU exhibits weak sequence similarity to the formerly hypothetical protein YiiL of Escherichia coli that has recently been characterized as an L-rhamnose mutarotase. To characterize RhaU further, a His-tagged variant of the protein was prepared and subjected to mass spectrometry analysis, confirming the subunit size and demonstrating its dimeric structure. After crystallization, the structure was refined to a 1.6-Å resolution to reveal a dimer in the asymmetric unit with a very similar structure to that of YiiL. Soaking a RhaU crystal with L-rhamnose resulted in the appearance of β-L.-rhamnose in the active site. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Publisher version (URL)http://dx.doi.org/10.1128/JB.01120-07
URIhttp://hdl.handle.net/10261/110041
DOI10.1128/JB.01120-07
Identifiersdoi: 10.1128/JB.01120-07
issn: 0021-9193
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