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dc.contributor.authorChavarría, Teresa-
dc.contributor.authorBaleriola, Jimena-
dc.contributor.authorMayordomo, Raquel-
dc.contributor.authorPablo, Flora de-
dc.contributor.authorDe la Rosa, Enrique J.-
dc.date.issued2013-
dc.identifier.citationThe Scientific World Journal (2013),ID 627240es_ES
dc.identifier.issn2356-6140-
dc.identifier.urihttp://hdl.handle.net/10261/110001-
dc.description9 p.-4 fig.es_ES
dc.description.abstractOrchestrated proliferation, differentiation, and cell death contribute to the generation of the complex cytoarchitecture of the central nervous system, including that of the neuroretina. However, few studies have comprehensively compared the spatiotemporal patterns of these 3 processes, or their relative magnitudes. We performed a parallel study in embryonic chick and mouse retinas, focusing on the period during which the first neurons, the retinal ganglion cells (RGCs), are generated. The combination of in vivo BrdU incorporation, immunolabeling of retinal whole mounts for BrdU and for the neuronal markers Islet1/2 and βIII-tubulin, and TUNEL allowed for precise cell scoring and determination the spatiotemporal patterns of cell proliferation, differentiation, and death. As predicted, proliferation preceded differentiation. Cell death and differentiation overlapped to a considerable extent, although the magnitude of cell death exceeded that of neuronal differentiation. Precise quantification of the population of recently born RGCs, identified by BrdU and βIII-tubulin double labeling, combined with cell death inhibition using a pan-caspase inhibitor, revealed that apoptosis decreased this population by half shortly after birth. Taken together, our findings provide important insight into the relevance of cell death in neurogenesis.es_ES
dc.description.sponsorshipThis work was supported by the Ministerio de Ciencia e Innovación, Spain (Grants SAF2007-66175 and SAF2010-21879 to EJdelaR and BFU 2007-61055 to FdeP).es_ES
dc.language.isoenges_ES
dc.publisherHindawi Publishing Corporationes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.titleEarly neural cell death is an extensive, dynamic process in the embryonic chick and mouse retinaes_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1155/2013/627240-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1155/2013/627240es_ES
dc.identifier.e-issn1537-744X-
dc.rights.licensehttp://creativecommons.org/licenses/by/3.0/es_ES
dc.relation.csices_ES
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