English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/109946
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 6 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Hydration Induced Stress on DNA Monolayers Grafted on Microcantilevers

Autor Domínguez, Carmen M.; Kosaka, Priscila M. ; Mokry, Guillermo; Pini, Valerio ; Malvar, Óscar ; Rey Franco, Mercedes del ; Ramos Vega, Daniel ; San Paulo, Álvaro ; Tamayo de Miguel, Francisco Javier ; Calleja, Montserrat
Fecha de publicación 22-ago-2014
EditorAmerican Chemical Society
Citación Langmuir 30(36): 10962–10969 (2014)
ResumenSurface tethered single-stranded DNA films are relevant biorecognition layers for oligonucleotide sequence identification. Also, hydration induced effects on these films have proven useful for the nanomechanical detection of DNA hybridization. Here, we apply nanomechanical sensors and atomic force microscopy to characterize in air and upon varying relative humidity conditions the swelling and deswelling of grafted single stranded and double stranded DNA films. The combination of these techniques validates a two-step hybridization process, where complementary strands first bind to the surface tethered single stranded DNA probes and then slowly proceed to a fully zipped configuration. Our results also demonstrate that, despite the slow hybridization kinetics observed for grafted DNA onto microcantilever surfaces, ex situ sequence identification does not require hybridization times typically longer than 1 h, while quantification is a major challenge.
Versión del editorhttp://dx.doi.org/10.1021/la501865h
URI http://hdl.handle.net/10261/109946
DOI10.1021/la501865h
ISSN0743-7463
E-ISSN1520-5827
Aparece en las colecciones: (IMN-CNM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
DNA_monolayers_Dominguez.pdf1,14 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.