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Hydration Induced Stress on DNA Monolayers Grafted on Microcantilevers

AuthorsDomínguez, Carmen M.; Kosaka, Priscila M. ; Mokry, Guillermo; Pini, Valerio ; Malvar, Óscar ; Rey Franco, Mercedes del ; Ramos Vega, Daniel ; San Paulo, Álvaro ; Tamayo de Miguel, Francisco Javier ; Calleja, Montserrat
Issue Date22-Aug-2014
PublisherAmerican Chemical Society
CitationLangmuir 30(36): 10962–10969 (2014)
AbstractSurface tethered single-stranded DNA films are relevant biorecognition layers for oligonucleotide sequence identification. Also, hydration induced effects on these films have proven useful for the nanomechanical detection of DNA hybridization. Here, we apply nanomechanical sensors and atomic force microscopy to characterize in air and upon varying relative humidity conditions the swelling and deswelling of grafted single stranded and double stranded DNA films. The combination of these techniques validates a two-step hybridization process, where complementary strands first bind to the surface tethered single stranded DNA probes and then slowly proceed to a fully zipped configuration. Our results also demonstrate that, despite the slow hybridization kinetics observed for grafted DNA onto microcantilever surfaces, ex situ sequence identification does not require hybridization times typically longer than 1 h, while quantification is a major challenge.
Publisher version (URL)http://dx.doi.org/10.1021/la501865h
Appears in Collections:(IMN-CNM) Artículos
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