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qPCR for Quantitative Detection and Typification of Dairy Bacteriophages

AuthorsRío Lagar, Beatriz del CSIC ORCID
Issue DateSep-2013
CitationqPCR & Digital PCR Congress (2013)
AbstractIn the Dairy Industry, members of the Lactic Acid Bacteria (LAB) group are the most important microorganisms used as starter cultures in the manufacture of milk fermented products, as cheese or yogurt. Starter cultures are responsible for the milk fermentation and confer characteristic flavors and textures to the final products. However, lytic bacteriophages present on the dairy environment can infect and kill the LAB used as starter cultures, which leads the delay and even total failure of the fermentative process. In fact, lytic bacteriophages have been pointed as the leading cause of fermentation failure during the manufacture of fermented milk products and as responsible of the largest-economic losses of the Dairy Industry. To avoid problems associated to bacteriophages, fast and reliable detection methods are needed for the early detection of infective bacteriophages in the milk or at any point during the fermentation procedure. We have developed methods based on the real-time quantitative PCR (qPCR) to quickly (30 minutes) detect and quantify bacteriophages that infect Streptococcus thermophilus and Lactobacillus delbrueckii, the two LAB species used as starter cultures of yogurt, a dairy product in which phages are particularly fastidious. The low quantification limits of the methods allow the detection of 102 bacteriophages in one microliter of milk, without any previous sample treatment. In addition, the PCR developed methods allow the typification of the detected phages. The rapid quantification and identification of potentially infective bacteriophages in milk is a valuable tool for dairy factories, as they can make fast decisions concerning the destination of the contaminated milk towards processes that are not affected by the detected bacteriophages and even guide a rational rotation of the starter cultures based on their host range.
DescriptionTrabajo presentado en el qPCR & Digital PCR Congress (Developments & Potential of qPCR & dPCR as a Tool for Progressing Molecular Biology Research), celebrado en Lyon (Francia) el 9 y 10 de septiembre de 2013.
Appears in Collections:(IPLA) Comunicaciones congresos
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