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dc.contributor.authorGómez-Casado, C.-
dc.contributor.authorGarrido-Arandia, M.-
dc.contributor.authorGamboa, P.-
dc.contributor.authorBlanca-López, N.-
dc.contributor.authorCanto, G.-
dc.contributor.authorVarela, Javier-
dc.contributor.authorCuesta-Herranz, J.-
dc.contributor.authorPacios, Luis F.-
dc.contributor.authorDíaz-Perales, Araceli-
dc.contributor.authorTordesillas, L.-
dc.date.accessioned2015-01-20T12:20:59Z-
dc.date.available2015-01-20T12:20:59Z-
dc.date.issued2013-
dc.identifier.citationClinical and Developmental Immunology 2013, ID 385615, 12 pageses_ES
dc.identifier.issn1740-2522-
dc.identifier.urihttp://hdl.handle.net/10261/109542-
dc.description13 p.-5 fig.-1 tab.es_ES
dc.description.abstractNowadays, treatment of food allergy only considered the avoidance of the specific food. However, the possibility of cross-reactivity makes this practice not very effective. Immunotherapy may exhibit as a good alternative to food allergy treatment. The use of hypoallergenic molecules with reduced IgE binding capacity but with ability to stimulate the immune system is a promising tool which could be developed for immunotherapy. In this study, threemutants of Pru p 3, the principal allergen of peach,were produced based on the described mimotope and T cell epitopes, by changing the specific residues to alanine, named as Pru p 3.01, Pru p 3.02,and Pru p 3.03. Pru p 3.01 showed very similar allergenic activity as the wild type by in vitro assays. However, Pru p 3.02 and Pru p 3.03 presented reduced IgE binding with respect to the native form, by in vitro, ex vivo, and in vivo assays. In addition, Pru p 3.03 had affected the IgG4 binding capacity and presented a random circular dichroism, which was reflected in the nonrecognition by specific antibodies anti-Pru p 3. Nevertheless, both Pru p 3.02 and Pru p 3.03 maintained the binding to IgG1 and their ability to activate T lymphocytes.Thus, Pru p 3.02 and Pru p 3.03 could be good candidates for potential immunotherapy in peach-allergic patients.es_ES
dc.description.sponsorshipFinancial support from the Ministerio de Ciencia e Innovación (Grant BIO2009-07050) and the Instituto de Salud Carlos III, RETICS 2007 (RD07/0064/03), Spain, are acknowledged. C. Gómez-Casado and L. Tordesillas were supported by training grants from the Spanish Government (FPI and FPU programmes, MEC, resp.).es_ES
dc.language.isoenges_ES
dc.publisherHindawi Publishing Corporationes_ES
dc.rightsopenAccesses_ES
dc.titleAllergenic characterization of new mutant forms of Pru p 3 as new immunotherapy vaccineses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1155/2013/385615-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1155/2013/385615es_ES
dc.identifier.e-issn1740-2530-
dc.rights.licensehttp://creativecommons.org/licenses/by-nc/3.0/es_ES
dc.relation.csices_ES
dc.identifier.pmid24324505-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeartículo-
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