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Allergenic characterization of new mutant forms of Pru p 3 as new immunotherapy vaccines

AutorGómez-Casado, C.; Garrido-Arandia, M.; Gamboa, P.; Blanca-López, N.; Canto, G.; Varela, Javier ; Cuesta-Herranz, J.; Pacios, Luis F.; Díaz-Perales, Araceli; Tordesillas, L.
Fecha de publicación2013
EditorHindawi Publishing Corporation
CitaciónClinical and Developmental Immunology 2013, ID 385615, 12 pages
ResumenNowadays, treatment of food allergy only considered the avoidance of the specific food. However, the possibility of cross-reactivity makes this practice not very effective. Immunotherapy may exhibit as a good alternative to food allergy treatment. The use of hypoallergenic molecules with reduced IgE binding capacity but with ability to stimulate the immune system is a promising tool which could be developed for immunotherapy. In this study, threemutants of Pru p 3, the principal allergen of peach,were produced based on the described mimotope and T cell epitopes, by changing the specific residues to alanine, named as Pru p 3.01, Pru p 3.02,and Pru p 3.03. Pru p 3.01 showed very similar allergenic activity as the wild type by in vitro assays. However, Pru p 3.02 and Pru p 3.03 presented reduced IgE binding with respect to the native form, by in vitro, ex vivo, and in vivo assays. In addition, Pru p 3.03 had affected the IgG4 binding capacity and presented a random circular dichroism, which was reflected in the nonrecognition by specific antibodies anti-Pru p 3. Nevertheless, both Pru p 3.02 and Pru p 3.03 maintained the binding to IgG1 and their ability to activate T lymphocytes.Thus, Pru p 3.02 and Pru p 3.03 could be good candidates for potential immunotherapy in peach-allergic patients.
Descripción13 p.-5 fig.-1 tab.
Versión del editorhttp://dx.doi.org/10.1155/2013/385615
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