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Extraction of new bioactives from Neochloris oleoabundans using pressurized technologies and food grade solvents

AuthorsCastro-Puyana, M. ; Herrero, Miguel ; Mendiola, J. A. ; Suárez-Alvarez, Sonia; Cifuentes, Alejandro ; Ibáñez, Elena
Issue Date2013
CitationProSCiba 2013
AbstractIn the last years there has been an increasing interest in functional food ingredients due to their capacity to promote our health. Thus, one of the main areas in Food Science and Technology is the extraction and characterization of new bioactive compounds of natural origin. Considering the huge biodiversity of microalgae it is easy to understand that they represent one of the most important biological sources for novel biological compounds. In fact, it has been suggested that secondary metabolites produced by these organisms under certain extreme conditions (temperature, nutrients, UV-vis irradiation), provide with unique structures with important activities for human health such as antioxidant. As an example, beta-carotene and astaxanthin produced by Dunaliella salina and Haematococcus pluvialis respectively, have important application in the nutraceutical market. In the search of new bioactives from microalgae, another important aspect to be considered is the development of fast and environmental-friendly extraction processes to isolate the bioactive compounds. The purpose of this work was to develop a methodology to extract and characterize the carotenoids of N. Oleoabundans to demonstrate for the first time the potential of this microalga as a source of antioxidants. Thus, pressurized liquid extraction (PLE) was optimized by means of an experimental design considering food grade solvents such as ethanol and limonene; the chemical characterization of the extracts was carried out by means of HPLC-MS. The main carotenoids accumulated by the microalgae were lutein, cantaxanthin, zeaxanthin, or monoester and diester of astaxanthin.
DescriptionTrabajo presentado a la III Iberoamerican Conference on Supercritical Fluids celebrada en Cartagena de Indias (Colombia) del 1 al 5 de abril de 2013.
Publisher version (URL)http://www.prosciba2013.com/#
Appears in Collections:(CIAL) Comunicaciones congresos
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