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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10947

In vivo photosystem I reduction in thermophilic and mesophilic cyanobacteria: The thermal resistance of the process is limited by factors other than the unfolding of the partners

AutorDurán, Raúl V.; Hervás, Manuel ; Rosa, Miguel A. de la ; Navarro, José A.
Palabras claveCyanobacteria
Cytochrome c6
Laser flash spectroscopy
Fecha de publicación24-jun-2005
CitaciónBiochemical and Biophysical Research Communications 334(1): 170-175 (2005)
ResumenPhotosystem I reduction by plastocyanin and cytochrome c6 in cyanobacteria has been extensively studied in vitro, but much less information is provided on this process inside the cell. Here, we report an analysis of the electron transfer from both plastocyanin and cytochrome c6 to photosystem I in intact cells of several cyanobacterial species, including a comparative study of the temperature effect in mesophilic and thermophilic organisms. Our data show that cytochrome c6 reduces photosystem I by following a reaction mechanism involving complex formation, whereas the copper–protein follows a simpler collisional mechanism. These results contrast with previous kinetic studies in vitro. The effect of temperature on photosystem I reduction leads us to conclude that the thermal resistance of this process is determined by factors other than the proper stability of the protein partners.
Descripción6 pages, 3 figures, 2 tables.-- PMID: 15992773 [PubMed].-- Printed version published on Aug 19, 2005.
Versión del editorhttp://dx.doi.org/10.1016/j.bbrc.2005.06.070
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