English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/109468
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Fungal genomes mining to discover novel sterol esterases and lipases as catalysts

AuthorsBarriuso, Jorge ; Prieto, Alicia ; Martínez, María Jesús
In silico
Fungal genomics
Candida rugosa
Ophiostoma piceae
Issue Date2013
PublisherBioMed Central
CitationBMC Genomics 2013, 14:712
AbstractBackground: Sterol esterases and lipases are enzymes able to efficiently catalyze synthesis and hydrolysis reactions of both sterol esters and triglycerides and due to their versatility could be widely used in different industrial applications. Lipases with this ability have been reported in the yeast Candida rugosa that secretes several extracellular enzymes with a high level of sequence identity, although different substrate specificity. This versatility has also been found in the sterol esterases from the ascomycetes Ophiostoma piceae and Melanocarpus albomyces. Results: In this work we present an in silico search of new sterol esterase and lipase sequences from the genomes of environmental fungi. The strategy followed included identification and search of conserved domains from these versatile enzymes, phylogenetic studies, sequence analysis and 3D modeling of the selected candidates. Conclusions: Six potential putative enzymes were selected and their kinetic properties and substrate selectivity are discussed on the basis of their similarity with previously characterized sterol esterases/lipases with known structures.
Description8 p.-3 fig.-1 tab.
Publisher version (URL)http://www.biomedcentral.com/1471-2164/14/712
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Barriuso, J. BMC Genomics 2013.pdf1,35 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.