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Title

Identification of an extracellular infection-induced glyceraldehyde-3-phosphate dehydrogenase of the phytopathogenic proteobacterium Pseudomonas syringae pv tomato DC3000

AuthorsElkhalfi, Bouchra; Serrano, Aurelio ; Soukri, Abdelaziz
KeywordsInfective State
Gene Expression
MALDI-TOF
Bacterial Speak
RNA
Secretome
GAPDH
Issue Date2014
PublisherScientific Research Publishing
CitationAdvances in Bioscience and Biotechnology 5: 201- 208 (2014)
AbstractAccording to molecular biology, genomic and proteo- mic data, the phytopathogenic gamma-proteobacte- rium Pseudomonas syringae pv. tomato DC3000 pro-duces a number of proteins that may promote infec- tion and draw nutrients from plants. Remarkably, P. syringae DC3000 strain possesses three paralogous gap genes encoding glyceraldehyde-3-phosphate dehy- drogenase (GAPDH) enzymes with different predic- ted molecular sizes and metabolic functions. As GAPDH was shown to be a virulence factor in other microbial pathogens, in the current study, we analyzed the ex-pression levels of each paralogous gap gene by real- time PCR to understand the actual impact of their protein products on P. syringae virulence. We found that all of them were strongly induced during the in-fection process. Nevertheless, proteomic analysis of cul- ture supernatants revealed that only Class I GAPDH1 encoded by the gap1 gene was identified as an extra-cellular protein in infective cells. These results strongly suggest that this GAPDH should play a role in the infective process, including its well-know en-zymatic function in the glycolytic metabolic pathway.
URIhttp://hdl.handle.net/10261/109261
DOI10.4236/abb.2014.53026
Identifiersdoi: 10.4236/abb.2014.53026
issn: 2156-8456
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