English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/109213
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
 |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Levels of ammonia, nitrite, and nitrate in alfalfa root nodules supplied with nitrate

Autor Becana Ausejo, Manuel ; Aparicio-Tejo, Pedro María ; Sánchez-Díaz, Manuel
Palabras clave Medicago sativa
ammonia
bacteroids
nitrate
nitrite
nodule cytosol
protein
proteolytic activity
Fecha de publicación 1985
EditorElsevier
Citación Journal of Plant Physiology 119 (4): 359–367 (1985)
ResumenProtein content, protease activity, and levels of NH4+, NO2- and NO3- were determined in the plant and bacteroid fractions of alfalfa (Medicago sativa L. cv. Aragon) nodules supplied with NO3- (0—200mM). Protein of the host cell cytosol represented approximately 70 % of the total nodule protein. The effect of NO3- on plant cytosolic protein closely paralleled that on bacteroid protein. Protein of both nodule fractions remained constant upon exposure to NO3- concentrations below 20 mM and was reduced by 30% above 20 mM NO3-. Patterns of proteolytic activity were inversely correlated to those of the corresponding protein for both bacteroids and cytosol. The nodule level of NH4+ was basically unaffected by increasing added NO3- whereas NO2- accumulated in both bacteroids and plant cytosol, although to a greater extent in the latter. Likewise, NO3- accumulated in the cytosol with much higher relative values than those of NO2-. Only trace or null amounts of NO3- were found in the corresponding bacteroids. Linear correlations either between bacteroidal and cytosolic NO2-, or between NO2- in both fractions and cytosolic NO3- levels were highly significant. The overall results indicate that the whole nodule capacity of NO2- reduction is not sufficient to avoid accumulation of NO2-, and support the view that NO2- plays a more important role than NH4+ or NO3- itself as inhibitor of nodule activity.
Descripción 9 Pags.
Versión del editorhttp://dx.doi.org/10.1016/S0176-1617(85)80103-6
URI http://hdl.handle.net/10261/109213
DOI10.1016/S0176-1617(85)80103-6
ISSN0176-1617
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
mod vers.aut pend.pdf20,83 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.