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Title: | Levels of ammonia, nitrite, and nitrate in alfalfa root nodules supplied with nitrate |
Authors: | Becana Ausejo, Manuel ![]() ![]() ![]() |
Keywords: | Medicago sativa ammonia bacteroids nitrate nitrite nodule cytosol protein proteolytic activity |
Issue Date: | 1985 |
Publisher: | Elsevier |
Citation: | Journal of Plant Physiology 119 (4): 359–367 (1985) |
Abstract: | Protein content, protease activity, and levels of NH4+, NO2- and NO3- were determined in the plant and bacteroid fractions of alfalfa (Medicago sativa L. cv. Aragon) nodules supplied with NO3- (0—200mM). Protein of the host cell cytosol represented approximately 70 % of the total nodule protein. The effect of NO3- on plant cytosolic protein closely paralleled that on bacteroid protein. Protein of both nodule fractions remained constant upon exposure to NO3- concentrations below 20 mM and was reduced by 30% above 20 mM NO3-. Patterns of proteolytic activity were inversely correlated to those of the corresponding protein for both bacteroids and cytosol. The nodule level of NH4+ was basically unaffected by increasing added NO3- whereas NO2- accumulated in both bacteroids and plant cytosol, although to a greater extent in the latter. Likewise, NO3- accumulated in the cytosol with much higher relative values than those of NO2-. Only trace or null amounts of NO3- were found in the corresponding bacteroids. Linear correlations either between bacteroidal and cytosolic NO2-, or between NO2- in both fractions and cytosolic NO3- levels were highly significant. The overall results indicate that the whole nodule capacity of NO2- reduction is not sufficient to avoid accumulation of NO2-, and support the view that NO2- plays a more important role than NH4+ or NO3- itself as inhibitor of nodule activity. |
Description: | 9 Pags. |
Publisher version (URL): | http://dx.doi.org/10.1016/S0176-1617(85)80103-6 |
URI: | http://hdl.handle.net/10261/109213 |
DOI: | http://dx.doi.org/10.1016/S0176-1617(85)80103-6 |
ISSN: | 0176-1617 |
Appears in Collections: | (EEAD) Artículos |
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