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Título: | Effects of water stress on enzymes of ammonia assimilation in root nodules of alfalfa (Medicago sativa) |
Autor: | Becana Ausejo, Manuel CSIC ORCID ; Aparicio-Tejo, Pedro María CSIC; Sánchez-Díaz, Manuel CSIC ORCID | Palabras clave: | Glutamate synthase Glutamine synthetase NAD+-glutamate dehydrogenase NADH-glutamate dehydrogenase |
Fecha de publicación: | ago-1984 | Editor: | John Wiley & Sons | Citación: | Physiologia Plantarum 61 (4): 653–657 (1984) | Resumen: | Protein content and activities of the enzymes glutamine synthetase (EC 6.3.1.2), NADH-glutamate synthase (EC 1.4.1.14), NADH-glutamate dehydrogenase (reductive amination (EC 1.4.1.2) and NAD+-glutamate dehydrogenase (oxidative deamination) (EC 1.4.1.2) from the plant fraction of root nodules of alfalfa (Medicago sativa L. cv. Aragon) were determined under water stress. Only NADH-glutamate synthase activity was inhibited during drought. The results indicate that the glutamine synthetase/NADH-glutamate synthase cycle was fully operational in alfalfa nodules of control or even mildly stressed plants when N2-fixation was not inhibited, but that the coupling between glutamine synthetase and NADH-glutamate synthase was lost as drought progressed. Patterns of glutamine synthetase and NADH-/NAD+-gluta-mate dehydrogenase activities reflect changes in ammonia content of nodules and/or availability of carbon substrates, and indicate that nodules maintain sufficient enzyme activity for ammonia assimilation throughout water stress. | Descripción: | 5 Pags. | Versión del editor: | http://dx.doi.org/10.1111/j.1399-3054.1984.tb05186.x | URI: | http://hdl.handle.net/10261/109202 | DOI: | 10.1111/j.1399-3054.1984.tb05186.x | ISSN: | 0031-9317 | E-ISSN: | 1399-3054 |
Aparece en las colecciones: | (EEAD) Artículos |
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