English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10919
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 13 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Purification, N-terminal sequencing and diagnostic value of the major antigens of Ornithodoros erraticus and O. moubata

Autor Baranda, José A.; Pérez Sánchez, Ricardo ; Oleaga, Ana ; Manzano Román, Raúl ; Encinas Grandes, Antonio
Palabras clave Ornithodoros
Antigens
Characterization
Serology
Fecha de publicación ene-2000
EditorElsevier
Citación Veterinary Parasitology 87(2-3): 193-206 (2000)
ResumenTo enhance the specificity and sensitivity of serological detection of swine exposed to Ornithodoros erraticus or O. moubata, we purified the 158, 186, 215 and 260 kDa antigens from the former species and the designated (owing to their MW and charge) 19C, 17A, 20A1 and 20A2 antigens of the latter by HPLC and gel electroelution methods. All the O. erraticus antigens share epitopes and are difficult to purify individually by reverse phase and ion-exchange chromatography due to their molecular similarity. Tested individually by ELISA, all of them give the same optical densities (OD) with anti-O. erraticus sera, and these ODs are always lower with anti-immature than with anti-adult sera. Although immature and adult specimens have the same antigens, immature forms induce more anti-carbohydrate antibodies than adults. This is the reason for the lower ODs of the anti-immature sera against purified antigens, since these latter antigens essentially react with anti-peptide antibodies (hence, increasing the specificity and sensitivity of the serology). The N-terminus of the 260 kDa antigen shows 80–90% similarity with the hemoglobin α-chain of many mammals. The antigens of O. moubata are proteins that are very different from one another and are, therefore, readily purified by ion exchange chromatography. The 20A1 antigen appears to be the most immunogenic and is recognized equally by anti-immature and anti-adult sera. This antigen does not give false positive reactions with the negative control sera analyzed and its N-terminus region shares 46.2% homology with the α-chain of the C3 component of rabbit complement.
Descripción 14 pages, 3 figures, 3 tables.-- PMID: 10622611 [PubMed].-- Available online on Nov 15, 1999.
Versión del editorhttp://dx.doi.org/10.1016/S0304-4017(99)00169-7
URI http://hdl.handle.net/10261/10919
DOI10.1016/S0304-4017(99)00169-7
ISSN0304-4017
Aparece en las colecciones: (IRNASA) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.