Characterization of antigenic polypeptides of the RNP, Sm and SS-B nuclear antigens from calf thymus

Abstract

Antinuclear autoantibodies are a hallmark of autoimmune diseases. The RNP, Sm and SS-B nuclear antigens from calf thymus in whole tissue, nuclear extracts and fractions have been studied by using different techniques including immunodiffusion, counterimmunoelectrophoresis and protein blotting. Such studies were done in order to obtain a precise characterization of the polypeptide components of those antigens. From our results it can be well established that: (a) one 69.8 Kd polypeptide (for whole tissue and nuclei) and a number of well-defined 32-38-Kd polypeptides (for nuclear extracts and ammonium sulfate fractions) show an antigenic character against anti-RNP sera; (b) anti-Sm sera from different patients show in all cases a variable component of antigenic polypeptides, including one 28.8, 29.7 Kd doublet and two singlets of 14.8 and 11.0 Kd; and (c) a 52.0-Kd SS-B antigenic polypeptide is found for whole tissue, which is gradually degraded in nuclei and nuclear extracts to a more stable 47.1-Kd polypeptide.